[Phylogeny of the genus Arundinaria based on nucleotide sequences of nrDNA ITS region].

ABSTRACT

The sequences of nrDNA regions of 17 species and Phyllostachys edulis (outgroup) sampled, which are of represent and type species for different taxa in the genus Arundinaria,were analyzed by PCR amplification and direct DNA sequencing. The phylogenetic trees generated from maximum parsimony analysis showed that the sampled bamboos were naturally monophletic, appearing that these species of the bamboos belong to the genus Arundinaria. The internal transcribed spacers (ITS) data indicated that the species were divided into two branches, one including A. oleosa, A. hsienchuensis, A. chino, A. amara, A. yixingensis, A. amabilis, A. fortunei, and A. pygmaea, the other including A. graminea, A. fargesii, A. faberi, A. hupehensis, Pseudosasa japonica cv. Tsutsumiana, P. japonica, Brachystachyum densiflorum, A. oedogonata, and A. sulcata. The result also showed that there was close relationship between A. graminea and A. fargesii, Pseudosasa japonica cv. Tsutsumiana and P. japonica, A. sulcata, Brachystachyum densiflorum and A. oedogonata, (99%, 100% and 82% boot-strap support respectively). Moreover, there was very close relationship between A. amabilis and A. hsienchuensis, indicating that A. amabilis belongs to the genus Arundinaria. It was shown in the phylogenetic tree that A. pygmaea and A. fortunei had close relationship, and were a sister branch to the bamboos of Pleioblastus.