CpG oligodeoxynucleotides stimulate IFN-gamma-inducible protein-10 production in human B cells.
J Endotoxin Res
; 10(6): 431-8, 2004.
Article
em En
| MEDLINE
| ID: mdl-15588427
ABSTRACT
Several classes of CpG oligodeoxynucleotides (ODNs) with different immune stimulatory profiles were recently identified the A-, B- and C-classes. In this study, we investigated the CpG-dependent stimulation of IFN-gamma-inducible protein 10 (IP-10 or CXCL10) in different human immune cell types. CpG ODNs induced IP-10 in monocytes, pDCs and in B cells. Purified B cells as well as RPMI 8226 cells responded to CpG stimulation by IP-10 production. Treatment with exogenous IFN-alpha2b sensitized PBMCs, purified B cells as well as RPMI 8226 cells to respond more efficiently to stimulation with CpG ODNs by IP-10 production. IP-10 signaling could be directly stimulated via TLR9 in CpG-unresponsive HEK293 cells transfected with human TLR9 and an IP-10 reporter construct. Therefore, CpG-mediated IP-10 production is stimulated through IFN-alpha in cells that express the IFN-alpha receptor, a second pathway for IP-10 induction exists in TLR9-expressing B cells and pDCs where IP-10 is stimulated directly upon CpG-mediated TLR9 signaling. Our data provide a better understanding of the mechanisms through which CpG ODNs induce efficient Th1 responses.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligodesoxirribonucleotídeos
/
Linfócitos B
/
Adjuvantes Imunológicos
/
Ilhas de CpG
/
Quimiocinas CXC
Limite:
Humans
Idioma:
En
Revista:
J Endotoxin Res
Assunto da revista:
ANGIOLOGIA
/
BACTERIOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Alemanha