Evidence that arachidonic acid derived from neutrophils and prostaglandin E2 are associated with the induction of acute lung inflammation by lipopolysaccharide of Escherichia coli.
Inflamm Res
; 53(12): 658-63, 2004 Dec.
Article
em En
| MEDLINE
| ID: mdl-15654513
ABSTRACT
OBJECTIVE:
The involvement of arachidonic acid (AA) and PGE2 during the E. coli lipopolysaccharide (LPS)-induced acute lung injury was investigated.MATERIAL:
Adult male Wistar rats were used. For in vitro studies, rat neutrophils, bronchoalveolar lavage (BAL) fluid, and lug vascular endothelium were used, as described below. TREATMENT Rats were given an intratracheal injection of LPS (750 microg).METHODS:
Total and differential cell counts in BAL fluid; enzyme-linked immunoassay (ELISA) analyses of TNF-alpha, IL-1beta, LTB4 and PGE2 in BAL, and immunohistochemical detection of ICAM-1 on lung vascular endothelium were performed six h after LPS challenge. Fatty acid composition of blood neutrophils and plasma was analyzed by HPLC.RESULTS:
Rats instilled with LPS presented a sixty three-fold increase in the number of neutrophils in BAL (from 0.5 x 10(6) to 31.5 x 10(6) cells), accompanied by increased levels of TNF-alpha and IL-1beta (p < 0.001), and a three-fold increase in ICAM-1 expression on vascular endothelium. The content of AA in blood neutrophils was reduced by 50%, whereas the level of PGE2 in BAL was increased by 3.5 fold, without changes in the levels of LTB4.CONCLUSIONS:
These findings suggest that AA and PGE2 are associated with LPS challenge.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pneumonia
/
Dinoprostona
/
Lipopolissacarídeos
/
Ácido Araquidônico
/
Escherichia coli
/
Neutrófilos
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Inflamm Res
Assunto da revista:
ALERGIA E IMUNOLOGIA
/
PATOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND