[The characteristics of the action of gamma irradiation on the secondary structure of ribonuclease in vitro and the effect of oxygen on this process]. / Osobennosti deistviia gamma-oblucheniia na vtorichnuiu strukturu ribonukleazy in vitro i vliianie kisloroda na étot protsess.

ABSTRACT

The change in the secondary structure of ribonuclease after 60Co gamma irradiation with a dose of 1,000 Gy in 0.2% aqueous solution was estimated using the circular dichroism method. The beta structures were significantly changed, while other types of the secondary structure (alpha-helix and beta-turns) changed insignificantly. The secondary structure injury was also affected by oxygen. The data are attributed to characteristics of the secondary structure of this enzyme.