[Association between high-risk human papillomavirus DNA load and cervical intraepithelial lesion].
Zhonghua Liu Xing Bing Xue Za Zhi
; 25(11): 921-4, 2004 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-15769315
ABSTRACT
OBJECTIVE:
To determine the association between viral load of high risk type human papillomavirus (HR-HPV) and stage of cervical intraepithelial neoplasia (CIN) lesion.METHODS:
Cervical exfoliated cells were collected from 1997 women aged 35-45 in a cross-sectional screening study. HPV DNA was detected by hybrid capture 2 (HC2) system, and viral load was measured by the ratios of relative light units compared to standard positive control (RLU/PC). Log10RLU/PC were categorized into four groups negative (< 0), low viral load (0 - 1.12), medium viral load (1.13 - 2.23), and high viral load (2.24 - 3.37). Cervical lesions were diagnosed by biopsies as normal, CIN 1, CIN 2-3, and squamous cervical cancer (SCC). Association between HR-HPV and CINs were evaluated by unconditional multinomial logistic regression.RESULTS:
100% (12/12) SCC, 97.3% (72/74) of CIN 2-3, 58.3% (74/127) of CIN 1, and 11.5% (205/1784) of normal women were positive for HPV DNA. The median log10RLUs for the positive women with SCC, CIN 2-3, CIN 1 and in normal women were 2.60, 2.32, 2.18 and 1.18 respectively. The odds ratio (OR) between low viral load of HPV DNA and CIN 1 was 3.8 (1.9 - 7.3) while between high viral load and CIN 2-3 was OR=865.9 (200.1 - 3738.0) which showed that higher viral load could increase the risk of cervical lesions (P <0.001).CONCLUSION:
Both cervical cancer and CINs were highly influenced by HR-HPV viral load.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
DNA Viral
/
Neoplasias do Colo do Útero
/
Carga Viral
/
Infecções por Papillomavirus
Tipo de estudo:
Etiology_studies
/
Observational_studies
/
Prevalence_studies
/
Prognostic_studies
/
Risk_factors_studies
/
Screening_studies
Limite:
Adult
/
Female
/
Humans
/
Middle aged
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Liu Xing Bing Xue Za Zhi
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China