Genome-wide analysis of gene expression profiles associated with cell cycle transitions in growing organs of Arabidopsis.
Plant Physiol
; 138(2): 734-43, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-15863702
ABSTRACT
Organ growth results from the progression of component cells through subsequent phases of proliferation and expansion before reaching maturity. We combined kinematic analysis, flowcytometry, and microarray analysis to characterize cell cycle regulation during the growth process of leaves 1 and 2 of Arabidopsis (Arabidopsis thaliana). Kinematic analysis showed that the epidermis proliferates until day 12; thereafter, cells expand until day 19 when leaves reach maturity. Flowcytometry revealed that endoreduplication occurs from the time cell division rates decline until the end of cell expansion. Analysis of 10 time points with a 6k-cDNA microarray showed that transitions between the growth stages were closely reflected in the mRNA expression data. Subsequent genome-wide microarray analysis on the three main stages allowed us to categorize known cell cycle genes into three major classes constitutively expressed, proliferative, and inhibitory. Comparison with published expression data obtained from root zones corresponding to similar developmental stages and from synchronized cell cultures supported this categorization and enabled us to identify a high confidence set of 131 proliferation genes. Most of those had an M phase-dependent expression pattern and, in addition to many known cell cycle-related genes, there were at least 90 that were unknown or previously not associated with proliferation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ciclo Celular
/
Arabidopsis
/
Genoma de Planta
/
Perfilação da Expressão Gênica
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Plant Physiol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Bélgica