Improved "electrophoretic map" of breast cyst fluid proteins.
Cancer Detect Prev
; 16(2): 107-12, 1992.
Article
em En
| MEDLINE
| ID: mdl-1600519
ABSTRACT
Human breast cyst fluid (BCF) proteins were evaluated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) after protein denaturation in the presence or absence of 2-mercaptoethanol. Both electrophoretic techniques yielded characteristic and reproducible banding patterns and achieved a high degree of resolution. The known four major protein bands were identified; silver-staining procedure resulted in more discrete bands (at least 13). Crossed-immunoelectrophoresis analyses of BCF allowed an improved protein mapping of this pathological fluid, presenting the opportunity of simultaneous quantification and identification of at least 22 proteins. The clinical and biological potential of these electrophoretic methods for detecting BCF proteins is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Doença da Mama Fibrocística
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Cancer Detect Prev
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Itália