Cytotoxic T lymphocytes against the antigen-processing-defective RMA-S tumor cell line.
Eur J Immunol
; 22(6): 1639-42, 1992 Jun.
Article
em En
| MEDLINE
| ID: mdl-1601045
ABSTRACT
RMA-S is an antigen processing-defective cell line, obtained from a Rauscher virus-induced tumor. The cells express only a low level of cell surface major histocompatibility complex (MHC) class I molecules, which are supposed to be devoid of internally derived antigenic peptides. We investigated Rauscher virus expression and Rauscher peptide presentation to virus-specific cytotoxic T lymphocytes (CTL) by this cell line. Viral proteins are expressed properly, both intracellularly and at the cell surface of RMA-S. Rauscher peptides are presented to virus-specific CTL in the groove of both the class I H-2Kb and Db molecules, but at a low level. Culture of RMA-S cells at room temperature increases their susceptibility to CTL. The RMA-S defect thus affects, but not totally abrogates, Rauscher peptide presentation by MHC class I molecules via the endogenous pathway. This indicates that the RMA-S antigen processing deficit is not absolute.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Linfócitos T Citotóxicos
/
Imunidade Celular
/
Reações Antígeno-Anticorpo
Limite:
Animals
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Holanda