Signaling pathways of hypocretin-1 actions on pyramidal neurons in the rat prefrontal cortex.
Neuroreport
; 16(13): 1529-33, 2005 Sep 08.
Article
em En
| MEDLINE
| ID: mdl-16110284
ABSTRACT
We have investigated the direct excitatory effects of hypocretin-1 on acutely isolated prefrontal cortical pyramidal neurons and explored the signaling mechanisms of these actions. Puff application of hypocretin-1 caused an excitation in the recorded neurons. These effects of hypocretin-1 were abolished by a phospholipase C inhibitor D609, demonstrating that phospholipase C mediates the actions of hypocretin-1. A specific protein kinase C inhibitor, bisindolylmaleimide II, blocked the excitatory actions of hypocretin-1, suggesting that protein kinase C plays a key role. Finally, protein kinase A inhibitor applied intracellularly did not affect the responses. These results indicate that hypocretin-1 excites prefrontal neurons by activation of phospholipase C and protein kinase C pathways, but not protein kinase A.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neuropeptídeos
/
Transdução de Sinais
/
Córtex Pré-Frontal
/
Células Piramidais
/
Peptídeos e Proteínas de Sinalização Intracelular
Limite:
Animals
Idioma:
En
Revista:
Neuroreport
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
China