Affinity chromatography of porcine pepsin A using quinolin-8-ol as ligand.
J Chromatogr A
; 1084(1-2): 108-12, 2005 Aug 19.
Article
em En
| MEDLINE
| ID: mdl-16114243
ABSTRACT
Stationary phase containing quinolin-8-ol immobilized on macroporous methacrylate support for the affinity chromatography of porcine pepsin A is described. Optimized chromatographic conditions for separation of porcine pepsin A on this stationary phase were found investigating the influence of pH, concentration, ionic strength and chemical composition of the used mobile phases. The stationary phase shows a good reproducibility of chromatographic analyses (relative standard deviation, +/-2%), a high recovery (ca. 93%) and a satisfactory capacity (13 mg pepsin A/1 mL stationary phase) for porcine pepsin A. The obtained findings confirm the applicability of affinity chromatography on the stationary phase with immobilized quinolin-8-ol to the isolation and determination of porcine pepsin A.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Afinidade
/
Pepsina A
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
República Tcheca