Methods for measuring type I collagen synthesis in vitro.
Methods Mol Med
; 117: 129-40, 2005.
Article
em En
| MEDLINE
| ID: mdl-16118450
ABSTRACT
The excess accumulation of type I collagen within tissues leads to organ dysfunction and occurs as a result of an imbalance between synthesis and degradation. This chapter outlines several methods to assess the in vitro production of type I collagen that are employed in our laboratory. We describe Western immunoblotting of intact alpha1(I) collagen using antibodies directed to alpha1(I) collagen amino and carboxyl propeptides. The measurement of alpha1(I) collagen mRNA levels using real-time polymerase chain reaction is then outlined. Finally, methods to determine the transcriptional regulation of alpha1(I) collagen using a nuclear run-on assay are described.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colágeno Tipo I
/
Biologia Molecular
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos