Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface.
Clin Oral Implants Res
; 16(5): 557-62, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-16164461
ABSTRACT
OBJECTIVES:
We studied the effect of various static magnetic fields (SMFs) on the adsorption of specific recombinant fibronectin (FN) peptide (hFNIII9-10) on the titanium surface. Furthermore, the responses of human osteosarcoma TE-85 cells in the SMF were observed. MATERIAL ANDMETHODS:
Various magnetic fields--1, 2, 3, 5, 7, 10 mT--were established by controlling the distance from Nd-Fe-B magnet to the disks. For FN adsorption experiment, machined titanium disks were incubated in 1 microM hFNIII9-10 at 37 degrees C overnight under magnetic field. The adsorbed hFNIII9-10 was measured as optical density (OD). For attachment study, TE-85 cells were incubated for 2 h on the hFNIII9-10 coated machined titanium disks and OD values were measured. As for proliferation study, titanium disks were incubated for 48 h after washing unattached cells in 2 h. The amount of proliferated TE-85 cell was also measured as OD value. Attachments of TE-85 cells under various intensities of magnetic field were observed using a scanning electron microscope.RESULTS:
The amount of adsorbed hFNIII9-10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE-85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group (P=0). Cell attachment in groups of 3, 7 mT showed no significant difference with that of control group. TE-85 cells were observed to attach through filopodia. Especially in 1 mT, flattened cells were predominant. In proliferation assay, 1 mT stimulated TE-85 cells showed significantly higher proliferation than those in 2, 3 and 7 mT (P=0).CONCLUSION:
Magnetic fields under 10 mT did not influence FN adsorption on the titanium surface. However, a significant effect was found on cell attachment and proliferation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Radiação
/
Titânio
/
Fibronectinas
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Clin Oral Implants Res
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Coréia do Sul