Extraction and isolation of mRNA from adult articular cartilage.
Anal Biochem
; 202(1): 89-95, 1992 Apr.
Article
em En
| MEDLINE
| ID: mdl-1621990
ABSTRACT
We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 M guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Cartilagem Articular
/
Proteínas da Matriz Extracelular
/
Colágeno
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Canadá