Differential role of lipid rafts in the functions of CD4+ and CD8+ human T lymphocytes with aging.
Cell Signal
; 18(7): 1017-30, 2006 Jul.
Article
em En
| MEDLINE
| ID: mdl-16236485
ABSTRACT
Lipid rafts are critical to the assembly of the T-cell receptor (TCR) signaling machinery. It is not known whether lipid raft properties differ in CD4+ and CD8+ T cells and whether there are age-related differences that may account in part for immune senescence. Data presented here showed that time-dependent interleukin-2 (IL-2) production was different between CD4+ and CD8+ T cells. The defect in IL-2 production by CD4+ T cells was not due to lower levels of expression of the TCR or CD28. There was a direct correlation between the activation of p56(Lck) and LAT and their association/recruitment with the lipid raft fractions of CD4+ and CD8+ T cells. p56Lck, LAT and Akt/PKB were weakly phosphorylated in lipid rafts of stimulated CD4+ T cells of elderly as compared to young donors. Lipid rafts undergo changes in their lipid composition (ganglioside M1, cholesterol) in CD4+ and CD8+ T cells of elderly individuals. This study emphasizes the differential role of lipid rafts in CD4+ and CD8+ T-cell activation in aging and suggests that the differential localization of CD28 may explain disparities in response to stimulation in human aging.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Envelhecimento
/
Linfócitos T CD4-Positivos
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Linfócitos T CD8-Positivos
/
Microdomínios da Membrana
Limite:
Adult
/
Aged
/
Humans
/
Middle aged
Idioma:
En
Revista:
Cell Signal
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Canadá