Determination of the novel hydroxymethyl glutaryl coenzyme A reductase inhibitor (RP 61969) and its dihydroxy acid hydrolysis product in human plasma by reversed-phase high-performance liquid chromatography.
J Chromatogr
; 575(2): 319-24, 1992 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-1629313
ABSTRACT
A method is described for the determination of the novel hydroxymethyl glutaryl coenzyme A reductase inhibitor RP 61969 (I) and its hydrolysis product, the dihydroxy acid RP 62420 (II), in human plasma. A structural isomer of I is used as internal standard. Both I and II were extracted from acidified plasma with diethyl ether. The dried residues were reconstituted in the high-performance liquid chromatography mobile phase and chromatographed on a 5 microns ODS2 column. The mobile phase used was aqueous dipotassium phosphate +tetra-n-butyl ammonium bromide (both 10 mM)-acetonitrile-methanol (60405, v/v). At a flow-rate of 1.5 ml min-1 and ambient temperature, the retention time of II is 3.5 min, that of the internal standard is 5 min, and that of I is 8 min. The method has been validated and applied to the assay of plasma samples resulting from a cell-plasma distribution experiment in human whole blood.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piranos
/
Cromatografia Líquida de Alta Pressão
/
Inibidores de Hidroximetilglutaril-CoA Redutases
/
Hidroxiácidos
/
Isoquinolinas
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Reino Unido