Fruit-specific V-ATPase suppression in antisense-transgenic tomato reduces fruit growth and seed formation.
Planta
; 223(6): 1272-80, 2006 May.
Article
em En
| MEDLINE
| ID: mdl-16322982
ABSTRACT
The vacuole is a large, multifunctional organelle related to the processes of cell expansion, solute accumulation, regulation of cytoplasmic ion concentrations, pH homeostasis and osmoregulation, which are directly or indirectly achieved by vacuolar H+-pumps vacuolar H+-ATPase (V-ATPase; EC 3.6.1.3) and vacuolar H+-pyrophosphatase (V-PPase; EC 3.6.1.1). In this study, we produced antisense-transgenic tomatoes (Lycopersicon esculentum L.) of the V-ATPase A subunit, which is under the control of the fruit-specific 2A11 promoter. One beta-glucuronidase (GUS)-transgenic line (GUS control) and seven A subunit antisense-transgenic lines were obtained. The amount of V-ATPase A subunit mRNA in fruit decreased in all antisense-transgenic lines, but in leaves showed no difference compared with the GUS control line and the nontransformant, suggesting that suppression of the V-ATPase A subunit by a 2A11 promoter is limited to fruit. The antisense-transgenic plants had smaller fruits compared with the GUS control line and the nontransformant. Surprisingly, fruits from the antisense-transgenic plants, except the fruit that still had relatively high expression of A subunit mRNA, had few seeds. Sucrose concentration in fruits from the antisense-transgenic plants increased, but glucose and fructose concentrations did not change. These results show the importance of V-ATPase, not only in fruit growth, but also in seed formation and in sugar composition of tomato fruit.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Geneticamente Modificadas
/
Solanum lycopersicum
/
ATPases Vacuolares Próton-Translocadoras
/
Frutas
Idioma:
En
Revista:
Planta
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Japão