Production of (10E,12Z)-conjugated linoleic acid in yeast and tobacco seeds.
Biochim Biophys Acta
; 1738(1-3): 105-14, 2005 Dec 30.
Article
em En
| MEDLINE
| ID: mdl-16324883
ABSTRACT
The polyenoic fatty acid isomerase from Propioniumbacterium acnes (PAI) was expressed in E. coli and biochemically characterized. PAI catalyzes the isomerization of a methylene-interrupted double bond system to a conjugated double bond system, creating (10E,12Z)-conjugated linoleic acid (CLA). PAI accepted a wide range of free polyunsaturated fatty acids as substrates ranging from 182 fatty acids to 226, converting them to fatty acids with two or three conjugated double bonds. For expression of PAI in yeast the PAI-sequence encoding 20 N-terminal amino acid residues was altered for optimal codon usage, yielding codon optimized PAI (coPAI). The percentage of 10,12-CLA of total esterified fatty acids was 8 times higher in yeast transformed with coPAI than in cells transformed with PAI. CLA was detected in amounts up to 5.7% of total free fatty acids in yeast transformed with coPAI but none was detected in yeast transformed with PAI. PAI or coPAI under the control of the constitutive CaMV 35S promoter or the seed-specific USP promoter was transformed into tobacco plants. CLA was only detected in seeds in coPAI-transgenic plants. The amount of CLA detected in esterified fatty acids was up to 0.3%, in free fatty acids up to 15%.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Sementes
/
Nicotiana
/
Isomerases de Ligação Dupla Carbono-Carbono
/
Ácidos Linoleicos Conjugados
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Alemanha