Mismatch tolerance by DNA polymerase Pol4 in the course of nonhomologous end joining in Saccharomyces cerevisiae.
Genetics
; 172(4): 2689-94, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16452137
ABSTRACT
In yeast, the nonhomologous end joining pathway (NHEJ) mobilizes the DNA polymerase Pol4 to repair DNA double-strand breaks when gap filling is required prior to ligation. Using telomere-telomere fusions caused by loss of the telomeric protein Rap1 and double-strand break repair on transformed DNA as assays for NHEJ between fully uncohesive ends, we show that Pol4 is able to extend a 3'-end whose last bases are mismatched, i.e., mispaired or unpaired, to the template strand.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
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Saccharomyces cerevisiae
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Fatores de Transcrição
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Proteínas de Saccharomyces cerevisiae
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Proteínas de Ligação a Telômeros
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DNA Polimerase Dirigida por DNA
Idioma:
En
Revista:
Genetics
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
França