Revision of the amino acid sequence of the smallest bc1 complex subunit: use of fast atom bombardment mass spectrometry and mass-analysed ion kinetic energy spectrum analysis.
Biol Mass Spectrom
; 20(4): 232-6, 1991 Apr.
Article
em En
| MEDLINE
| ID: mdl-1647218
ABSTRACT
We have isolated the smallest bc1 complex subunit from an acidic chloroform/methanol extract of bovine cardiac muscle. The identification of the polypeptide was made possible by classical Edman degradation and amino acid analysis. The measurement of its exact molecular weight by fast atom bombardment mass spectrometry (m/z 6519.8), the characterization of a tryptophan cleavage peptide and pepsic peptides by mass measurements and by mass-analysed ion kinetic energy spectrum analysis allow rectification of the amino acid sequence of the smallest bc1 complex subunit. We found a serine residue instead of Gln 22 and tryptophan residues in place of Ser 34 and Ser 38.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complexo III da Cadeia de Transporte de Elétrons
/
Miocárdio
Limite:
Animals
Idioma:
En
Revista:
Biol Mass Spectrom
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
França