Real-time PCR: housekeeping genes in the INS-1E beta-cell line.
Horm Metab Res
; 38(1): 8-11, 2006 Jan.
Article
em En
| MEDLINE
| ID: mdl-16477533
ABSTRACT
Investigation of gene expression is a developing area with several methods available. One method is quantitative PCR. A major pitfall in quantitative PCR is the normalisation procedure of the gene expression. Many experiments include a housekeeping gene, some use RNA concentration, and others use a geometric mean of several internal, stably expressed genes. This study demonstrates that real-time-PCR results differ with varying housekeeping genes and analysis protocols when applied to insulin-secreting INS-1E cells derived from the pancreas and stimulated by DEDTC (diethyldithiocarbamate, a zinc chelator) and GLP-1.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Perfilação da Expressão Gênica
/
Células Secretoras de Insulina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Horm Metab Res
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Dinamarca