The S2 accessory gene of equine infectious anemia virus is essential for expression of disease in ponies.
Virology
; 349(1): 22-30, 2006 May 25.
Article
em En
| MEDLINE
| ID: mdl-16503341
ABSTRACT
Equine infectious anemia virus (EIAV) is a macrophage-tropic lentivirus that persistently infects horses and causes a disease that is characterized by periodic episodes of fever, thrombocytopenia, and viremia. EIAV encodes only four regulatory/accessory genes, (tat, rev, ttm, and S2) and is the least genetically complex of all known lentiviruses. We sought to determine the role of the EIAV S2 accessory gene of EIAV by introducing mutations that would prevent S2 expression on the p19/wenv17 infectious molecular clone. Virus derived from the p19/wenv17 molecular clone is highly virulent and routinely fatal when given in high doses (J. Virol. 72 (1998) 483). In contrast, an S2 deletion mutant on the p19/wenv17 background is unable to induce acute disease and plasma virus loads were reduced by 2.5 to 4.0 logs at 15 days post-infection. The S2 deleted virus failed to produce any detectable clinical signs during a 5-month observation period. These results demonstrate that S2 gene expression is essential for disease expression of EIAV.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Anemia Infecciosa Equina
/
Vírus da Anemia Infecciosa Equina
/
Genes Virais
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos