Caspase 3, periodically expressed and activated at G2/M transition, is required for nocodazole-induced mitotic checkpoint.
Apoptosis
; 11(5): 765-71, 2006 May.
Article
em En
| MEDLINE
| ID: mdl-16532268
ABSTRACT
Caspases have been known for several years for their involvement in executing apoptosis, where unwanted or damaged cells are eliminated. Surprisingly, after analysis of the relevant data set from the Stanford microarray database, we noticed that the gene expression pattern for caspase 3, but not for caspase 1, 6, 7, 8, 9, or 10, undergoes periodic change in the HeLa cell cycle. In this study, we have demonstrated that caspase 3, but not other caspases, is upregulated and activated just prior to mitosis. Pretreatment of human hepatoma cells with a caspase 3 inhibitor z-DEVD-FMK, prior to the treatment with an antimicrotubule drug nocodazole, abrogates the mitotic arrest, suggesting that caspase 3 (or a caspase 3-like enzyme) might be involved in mitotic-spindle checkpoint. The studies not only characterize caspase 3 as a cell cycle-regulated protein, but also link the protein to nocodazole-dependent mitotic checkpoint, greatly expanding the understanding of caspase 3.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nocodazol
/
Fase G2
/
Caspases
/
Mitose
Limite:
Humans
Idioma:
En
Revista:
Apoptosis
Ano de publicação:
2006
Tipo de documento:
Article