Inhibition of adenylate cyclase by p-bromophenacyl bromide.
Biochem Pharmacol
; 42(10): 1941-6, 1991 Oct 24.
Article
em En
| MEDLINE
| ID: mdl-1660272
ABSTRACT
p-Bromophenacyl bromide (BPB) is an alkylating agent which has been used in biochemical studies as an inhibitor of phospholipase A2 activity. We report here that BPB irreversibly inhibited adenylate cyclase activity stimulated by hormones, forskolin, GppNHp, NaF, and cholera toxin. The action of BPB in S49 lymphoma cell membranes (wild type and cyc-) indicates that it can inhibit adenylate cyclase function in the absence of Gs. In the presence of Gs, however, inhibition of adenylate cyclase by BPB was enhanced, suggesting that BPB may covalently modify the catalytic protein on a site involved in activated catalytic functioning or critical to its interaction with Gs and/or additionally on the alpha s protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetofenonas
/
Alquilantes
/
Inibidores de Adenilil Ciclases
Limite:
Animals
Idioma:
En
Revista:
Biochem Pharmacol
Ano de publicação:
1991
Tipo de documento:
Article