Your browser doesn't support javascript.
loading
Determination of the inflammatory potential of bioaerosols from a duck-fattening unit by using a limulus amebocyte lysate assay and human whole blood cytokine response.
Zucker, B-A; Scharf, P; Kersten, C.
Afiliação
  • Zucker BA; Institute of Animal and Environmental Hygiene, Freie Universität Berlin, Fachbereich Veterinärmedizin, Luisenstrasse 56, 10117 Berlin, Germany. b_zucker@hotmail.com
Article em En | MEDLINE | ID: mdl-16629985
Inhalation of bioaerosols from animal houses can induce acute inflammatory reactions in the respiratory tract. Determination of the concentration of airborne endotoxins is widely used to characterize this risk. In this study, the activity of bioaerosol samples from a duck-fattening unit to induce interleukin-1beta (IL-1beta) in human blood and to react with Limulus Amebocyte Lysate (LAL) was investigated. The activity detected in the whole blood assay correlated well with the endotoxic activity found in the LAL assay (Spearmen's rho = 0.902). However in all samples, the inflammation-inducing potential was overestimated by the LAL assay. It is assumed that this overestimation could be, in part, a result of an overestimation of the inflammatory potential of endotoxins originating from Pseudomonadaceae by the LAL assay. Pseudomonadaceae were regularly isolated from the air of the duck-fattening unit. The results presented here indicate that the whole blood assay can be used besides the LAL assay as an additional method to characterize the inflammation-inducing potential of bioaerosols.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-1 / Microbiologia do Ar / Patos / Endotoxinas / Teste do Limulus Limite: Animals / Humans Idioma: En Revista: J Vet Med B Infect Dis Vet Public Health Assunto da revista: MEDICINA VETERINARIA / SAUDE PUBLICA Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Alemanha
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-1 / Microbiologia do Ar / Patos / Endotoxinas / Teste do Limulus Limite: Animals / Humans Idioma: En Revista: J Vet Med B Infect Dis Vet Public Health Assunto da revista: MEDICINA VETERINARIA / SAUDE PUBLICA Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Alemanha