Detection of induced anti-sperm antibodies by an improved enzyme-linked immunosorbent assay.
Int J Fertil
; 36(1): 48-56, 1991.
Article
em En
| MEDLINE
| ID: mdl-1672677
ABSTRACT
The enzyme-linked immunosorbent assay (ELISA) has been adapted for the detection of human anti-sperm antibodies (ASA). The use of peroxidase in the enzyme-anti-immunoglobulin conjugate, together with a nontoxic chromogenic substrate (MBTH-DMAB), renders the assay highly sensitive for detection of sperm surface antigens. This ELISA is highly reproducible, not only intra-assay (94% to 97%) but also interassay (80% to 96%). A great advantage of this ELISA is that microplates can be kept at -20 degrees C for months without any decrease in the response. New Zealand white rabbits showed a significant response to human sperm and tail fractions obtained by sonication. The sperm were extremely immunogenic, reaching titers of antisera of 1/10,000 or more. The results show that this ELISA is a good tool for the objective measurement of the anti-sperm response, and is significantly more sensitive than other techniques for anti-sperm antibody analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Anticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Int J Fertil
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Espanha