The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods.
Plant Methods
; 2: 12, 2006 Jun 26.
Article
em En
| MEDLINE
| ID: mdl-16800872
ABSTRACT
Non-invasive fluorophore-based protein interaction assays like fluorescence resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC, also referred to as "split YFP") have been proven invaluable tools to study protein-protein interactions in living cells. Both methods are now frequently used in the plant sciences and are likely to develop into standard techniques for the identification, verification and in-depth analysis of polypeptide interactions. In this review, we address the individual strengths and weaknesses of both approaches and provide an outlook about new directions and possible future developments for both techniques.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Plant Methods
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Alemanha