Application of packed column supercritical fluid chromatography to the analysis of barbiturates.

The analysis of barbiturates using packed column supercritical fluid chromatography (SFC) has been investigated. The separations were carried out on either an ODS bonded silica or polystyrene-divinylbenzene polymer column with carbon dioxide as the mobile phase and flame ionisation detection. All the barbiturates were strongly adsorbed on the ODS-silica column, but reasonable separations were obtained by using the polymer column.