Early legume responses to inoculation with Rhizobium sp. NGR234.
J Plant Physiol
; 164(6): 794-806, 2007 Jun.
Article
em En
| MEDLINE
| ID: mdl-16887234
ABSTRACT
Interactions between legumes and rhizobia are controlled by the sequential exchange of symbiotic signals. Two different techniques, 2D-PAGE electrophoresis and differential display were used to study the effects of rhizobial signals on legume development. Application of variously substituted lipo-oligo-saccharidic Nod-factors to roots of Vigna unguiculata resulted in changes in the phosphorylation patterns of microsomal proteins. Reliable amino-acid sequences were obtained for one Nod-factor enhanced protein which was highly homologous to the 57-kDa subunit from Arabidopsis thaliana vacuolar membrane H(+)-ATPase. Immuno-blotting techniques demonstrated that Nod-factors cause rapid and massive increases of this enzyme in treated roots, suggesting that H(+)-ATPases play symbiotic roles. Concomitantly, we used differential display (DD) techniques on mRNA isolated from root-hairs to analyse early root responses to NGR234. Significant matches of several DD clones to known sequences were found. Clone D2.62 was homologous to a multitude of receptor kinases including S receptor-like kinases of A. thaliana and clone D4.1 showed similarities to Lotus japonicus phosphatidylinositol transfer-like protein III and late nodulin 16. Independent confirmatory analyses of these differentially expressed clones indicated expression at very low levels.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rhizobium
/
Fabaceae
Idioma:
En
Revista:
J Plant Physiol
Assunto da revista:
BOTANICA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Suíça