Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex.
Biochemistry (Mosc)
; 71(7): 815-20, 2006 Jul.
Article
em En
| MEDLINE
| ID: mdl-16903837
ABSTRACT
We are the first to have isolated a protein (186 amino acid residues) encoded by the open reading frame adjacent to the end of the BspD6I nickase (N.BspD6I) gene. Cleavage of both DNA strands near the sequence recognized by nickase (5 -GAGTC/5 -GACTC) occurs when this protein is added to the reaction mixture containing N.BspD6I. The protein encoded by the open reading frame and the nickase are suggested to be subunits of heterodimeric restriction endonuclease R.BspD6I.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Enzimas de Restrição do DNA
/
Proteínas de Escherichia coli
/
Desoxirribonuclease I
Idioma:
En
Revista:
Biochemistry (Mosc)
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Federação Russa