[Determination of orlistat in health food for controlling body weight by high performance liquid chromatography/electrospray spectrometry].

ABSTRACT

OBJECTIVE: To establish a method of determining orlistat in health food by on line HPLC-UV-ESI/MS. METHODS: The separation was completed on an analytical Spherigel C8 column (5 microm, 200 mm x 4.6 mm) with acetonitrile (0.1% formic acid) water (0.1% formic acid) = 80 20 as mobile phase, the detection wavelength was 2003 mm. RESULTS: Good linearity between peak areas and concentrations was obtained during rag of 0.3 - 0.6 mg/ml. CONCLUSION: The method is simple and can be used to determine orlistat in the health food for controlling body weight accurately.