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The hepatitis C virus RNA 3'-untranslated region strongly enhances translation directed by the internal ribosome entry site.
Song, Yutong; Friebe, Peter; Tzima, Eleni; Jünemann, Christiane; Bartenschlager, Ralf; Niepmann, Michael.
Afiliação
  • Song Y; Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University Giessen, Friedrichstrasse 24, 35392 Giessen, Germany.
J Virol ; 80(23): 11579-88, 2006 Dec.
Article em En | MEDLINE | ID: mdl-16971433
ABSTRACT
The positive-strand RNA genome of the hepatitis C virus (HCV) is flanked by 5'- and 3'-untranslated regions (UTRs). Translation of the viral RNA is directed by the internal ribosome entry site (IRES) in the 5'-UTR, and subsequent viral RNA replication requires sequences in the 3'-UTR and in the 5'-UTR. Addressing previous conflicting reports on a possible function of the 3'-UTR for RNA translation in this study, we found that reporter construct design is an important parameter in experiments testing 3'-UTR function. A translation enhancer function of the HCV 3'-UTR was detected only after transfection of monocistronic reporter RNAs or complete RNA genomes having a 3'-UTR with a precise 3' terminus. The 3'-UTR strongly stimulates HCV IRES-dependent translation in human hepatoma cell lines but only weakly in nonliver cell lines. The variable region, the poly(U . C) tract, and the most 3' terminal stem-loop 1 of the highly conserved 3' X region contribute significantly to translation enhancement, whereas stem-loops 2 and 3 of the 3' X region are involved only to a minor extent. Thus, the signals for translation enhancement and for the initiation of RNA minus-strand synthesis in the HCV 3'-UTR partially overlap, supporting the idea that these sequences along with viral and possibly also cellular factors may be involved in an RNA 3'-5' end interaction and a switch between translation and RNA replication.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biossíntese de Proteínas / Replicação Viral / RNA Viral / Hepacivirus / Regiões 3' não Traduzidas Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biossíntese de Proteínas / Replicação Viral / RNA Viral / Hepacivirus / Regiões 3' não Traduzidas Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Alemanha