Rat myogenic c-mos cDNA: cloning sequence analysis and regulation during muscle development.
Oncogene
; 5(8): 1149-57, 1990 Aug.
Article
em En
| MEDLINE
| ID: mdl-1697408
ABSTRACT
We have isolated and sequenced a cDNA clone, homologous to the rat c-mos gene, from a cDNA library of rat skeletal muscles. The 3220 nucleotide cDNA clone codes for a protein of 339 amino acids (37.4 kDa). Both the nucleotide sequence and the deduced amino acid sequence show 60-90% overall homology to Xenopus, chicken, mouse and human mos. By Northern blot analysis, we detected two c-mos transcripts, one major of about 3.6 Kb long, and one minor of about 1.7 Kb long. These are differently regulated during the development of cardiac and skeletal muscles. By Western blot with two antibodies directed against two different portions of the mos protein, we observed in rat muscle two polypeptides of 43 kDa, and 75 kDa respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Regulação da Expressão Gênica
/
Proteínas Proto-Oncogênicas
/
Clonagem Molecular
/
Músculos
Limite:
Animals
Idioma:
En
Revista:
Oncogene
Assunto da revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
França