HPLC method for quantification and characterization of cholesterol and its oxidation products in eggs.
Lipids
; 41(6): 615-22, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16981439
ABSTRACT
A new method was developed for the simultaneous determination of cholesterol and its oxidation products in eggs, using HPLC with UV and refractive index (RI) detectors, and HPLC interfaced with atmospheric pressure chemical ionization coupled to MS (HPLC-APCI-MS). The best conditions for direct saponification of the sample and extraction of the non-saponifiable material were defined using complete factorial designs with central points. The method showed accuracy and precision with a detection limit between 0.002 and 0.079 microg/g. The oxides cholest-5-ene-3beta,20alpha-diol and cholest-5-ene-3beta,25-diol identified by HPLC-UV-RI were not confirmed by HPLC-APCI-MS.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colesterol
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Cromatografia Líquida de Alta Pressão
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Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Idioma:
En
Revista:
Lipids
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Brasil