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The suppressant effect of GEA3162 on spontaneous serotonin release from human colonic mucosa in vitro.
Kojima, Shu-Ichi; Uchida, Kohsuke; Sasaki, Kinro; Sunagawa, Masakatu; Ohno, Yasuo; Kamikawa, Yuichiro.
Afiliação
  • Kojima S; Department of Pharmacology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan. s-kojima@dokkyomed.ac.jp
Eur J Pharmacol ; 550(1-3): 162-5, 2006 Nov 21.
Article em En | MEDLINE | ID: mdl-17022966
The effect of a lipophilic nitric oxide (NO)-releasing compound 5-amino-3-(3,4-dichlorophenyl) 1,2,3,4-oxatriazolium (GEA3162) on the spontaneous release of 5-hydroxytryptamine (5-HT) from human colonic mucosa was investigated in vitro. In the presence of tetrodotoxin, spontaneous outflow of 5-HT from the human colonic mucosa was measured by high-performance liquid chromatography with electrochemical detection. GEA3162 concentration-dependently suppressed the 5-HT outflow, but neither the NO-activated soluble guanylate cyclase inhibitor 1H-[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) nor peroxynitrite scavenger ebselen affected the suppressant effect of GEA3162. Moreover, neither the L-type calcium channel blocker nicardipine, NO synthase inhibitor l-N(G)-nitroarginine methyl ester nor guanylate cyclase activator guanylin affected the spontaneous 5-HT outflow. These results indicate that human colonic mucosa is capable of eliciting tetrodotoxin-resistant and nicardipine-insensitive 5-HT release, and that GEA3162 can suppress the 5-HT release via an action on colonic mucosa through mechanism independent of ODQ-sensitive cyclic GMP system or peroxynitrite generation.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triazóis / Serotonina / Colo / Doadores de Óxido Nítrico / Mucosa Intestinal Idioma: En Revista: Eur J Pharmacol Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Japão País de publicação: Holanda
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triazóis / Serotonina / Colo / Doadores de Óxido Nítrico / Mucosa Intestinal Idioma: En Revista: Eur J Pharmacol Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Japão País de publicação: Holanda