The use of DNA microarray technology for detection and genetic characterisation of chlamydiae.
Dev Biol (Basel)
; 126: 203-10; discussion 326-7, 2006.
Article
em En
| MEDLINE
| ID: mdl-17058496
ABSTRACT
Due to its highly parallel approach, DNA microarray technology opens up new possibilities that may be particularly beneficial for laboratory diagnosis of infectious diseases. We developed a microarray assay for detection and differentiation of all currently defined chlamydial species belonging to the genera Chlamydia and Chlamydophila using the ArrayTube system, which we found to be particularly user-friendly and economical. The test includes PCR amplification of a 23S rDNA target region with concurrent biotinylation and subsequent hybridisation in the ArrayTube, a micro-reaction tube carrying the microarray chip on the bottom. In addition to high specificity, the assay was shown to allow detection and genetic characterisation of single PCR-amplifiable target DNA copies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Chlamydia
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Chlamydophila
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Dev Biol (Basel)
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Alemanha