Detection of infectious salmon anaemia virus by real-time nucleic acid sequence based amplification.
Dis Aquat Organ
; 72(2): 107-13, 2006 Oct 17.
Article
em En
| MEDLINE
| ID: mdl-17140133
ABSTRACT
We have developed a real-time nucleic acid sequence based amplification (NASBA) procedure for detection of infectious salmon anaemia virus (ISAV). Primers were designed to target a 124 nucleotide region of ISAV genome segment 8. Amplification products were detected in real-time with a molecular beacon (carboxyfluorescin [FAM]-labelled and methyl-red quenched) that recognised an internal region of the target amplicon. Amplification and detection were performed at 41 degrees C for 90 min in a Corbett Research Rotorgene. The real-time NASBA assay was compared to a conventional RT-PCR for ISAV detection. From a panel of 45 clinical samples, both assays detected ISAV in the same 19 samples. Based on the detection of a synthetic RNA target, the real-time NASBA procedure was approximately 100x more sensitive than conventional RT-PCR. These results suggest that real-time NASBA may represent a useful diagnostic procedure for ISAV.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Orthomyxoviridae
/
Salmo salar
/
Replicação de Sequência Autossustentável
/
Isavirus
/
Doenças dos Peixes
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Dis Aquat Organ
Assunto da revista:
BIOLOGIA
/
MEDICINA VETERINARIA
/
MICROBIOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Reino Unido