[Antigenic diversity of asexual schizont antigen GP195 of Plasmodium falciparum isolates from China].

ABSTRACT

The antigenic diversity of asexual merozoite surface antigen precursor gp195 among 6 isolates of Plasmodium falciparum from Hainan and Jiangsu provinces of China was investigated with a panel of murine anti-gp195 McAbs by indirect immunofluorescence assay and Western blotting. The results show that all isolates express certain strain-common epitopes of gp195, and at the same time express or lack some strain-specific epitopes. The 6 isolates can be divided into two major gp195 serotypes according to the reactivity patterns with the panel of anti-gp195 McAbs. Remarkable molecular weight difference in gp195 was found among 4 isolates of P. falciparum in which the Jiangsu isolate possesses a large size of gp195 (210kDa) than the other 3 Hainan isolates (less than or equal to 200kDa). The polymorphism of gp195 and it's impact on the development of antimalarial vaccine is discussed.