Highly efficient strand invasion by peptide nucleic acid bearing optically pure lysine residues in its backbone.
Nucleic Acids Symp Ser (Oxf)
; (50): 109-10, 2006.
Article
em En
| MEDLINE
| ID: mdl-17150841
ABSTRACT
Chiral PNA monomers (PNA = peptide nucleic acid), in which nucleobases are attached to N-(aminoethyl)-D-lysine, were introduced to PNAs bearing pseudo-complementary nucleobases (2,6-diaminopurine and 2-thiouracil). When these highly cationic PNAs targeted double-stranded DNA, they invaded there much more efficiently than conventional pseudo-complementary PNAs composed of achiral PNA monomers. Although introduction of N-(aminoethyl)-D-lysine backbone was effective for promotion of strand invasion, L-isomer never promote it. Simple incorporation of lysine groups to the termini of PNA was also ineffective, indicating that introduction of positive charges into PNA backbone is important. Even highly G-C rich sequence, which conventional pseudo-complementary PNAs never invade, was successfully targeted based on this strategy.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Ácidos Nucleicos Peptídicos
/
Lisina
Idioma:
En
Revista:
Nucleic Acids Symp Ser (Oxf)
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Japão