Immunohistological analysis of immune cells in blistering skin lesions.
J Clin Pathol
; 60(1): 62-71, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17213348
ABSTRACT
BACKGROUND:
Bullous skin lesions are characterised by the presence of intraepidermal or subepidermal bullae. Although inflammatory cell infiltrate is a constant feature in these lesions, their immunophenotypic characterisation is still incomplete.AIM:
To determine whether the development of bullous skin diseases is associated with changes in the inflammatory cell infiltrate. MATERIALS ANDMETHODS:
34 cases representing lesions with both intraepidermal and subepidermal bullae were examined using immunoperoxidase staining methods and antibodies targeting antigens for histiocytes (CD68), B cells (CD20+), T cells (CD3+), T cells with cytotoxic potential (T cell intracellular associated antigen, TIA1+) and activity (granzyme B, GRB+). The adjacent normal skin (lesions) and an additional five cases of normal skin were also examined (controls).RESULTS:
The transition from normal skin to lesional skin (lesions with intraepidermal and subepidermal bullae) was associated with a significant increase (p< or =0.05) in the density of total inflammatory cell infiltrate, CD68+ cells, CD3+ T lymphocytes, CD20+ B lymphocytes, TIA1+ -resting cytotoxic T cells and GRB+ T cells with cytotoxic activity.CONCLUSIONS:
The increase in inflammatory cell infiltrate during the transition from normal to lesional skin may reflect the presence of an increased antigenicity of the lesional cells or a response to some basement membrane components. CD68+ and CD3+ cells, especially the resting cytotoxic ones, achieved numerical dominance in these lesions. Cell-mediated immunity seems to have critical role in the development of these lesions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dermatopatias Vesiculobolhosas
Limite:
Adolescent
/
Adult
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Aged
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Child
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Child, preschool
/
Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Clin Pathol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Egito