[Relationship between CO II gene of mtDNA of Lucilia sericata and latitude interval].

ABSTRACT

OBJECTIVE: To deduce the region that the geographical species of Lucilia sericata come from and determine the scene of crime (SOC) based on the gene analysis of mtDNA CO II. METHODS: A 635 bp region for CO II of 4 Lucilia sericata (belong to 2 geographical species) were collected and sequenced, compared with the data of GenBank. A neighbour-joining tree with the Tamura and Nei model was constructed by MEGA2.1 package. The number of inherit intervals of inner-species were analyzes by Kimura's two-parameter model and used for construction the relationships between hereditary and latitude interval by SPSS10.5 soft. RESULTS: It showed that they had the relationships between inherit and latitude interval for the 8 geographical species of Lucilia sericata for CO II. CONCLUSION: This method can be the evidence deducing the region that the geographical species of Lucilia sericata come from and further to determine the scene of crime (SOC).