[The mechanism and significance of cellular signal transduction in kidney glomerular mesangial cells of rat with obstructive jaundice].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 38(1): 116-8, 2007 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-17294743
ABSTRACT
OBJECTIVE:
To study the mechanism of ceramide-induced cellular signal transduction and its effect on renal injury.METHODS:
Sixty male Sprague-Dawley rats were randomly assigned to four groups (n = 15) control group, sham operation group, experiment group one and experiment group two. Except the control and the sham operation group, the other two experiment groups underwent the common bile duct bound to form the rat model of obstructive jaundice. The renal glomerular mesangial cells (GMC) were cultured primarily. After GMC were given different stimulating factors, we performed the measurement of the PLD activity and the GMC apoptosis analyzed by flow cytometry.RESULTS:
The biological activity of PC-PLD was significantly decreased by TNF-alpha and ceramide (P< 0.05). On the other hand, the GMC apoptosis was induced by TNF-alpha and ceramide (P <0. 05). However, other opposite results were obtained in addition of ceramide inhibitor to GMC.CONCLUSION:
TNF-alpha can induce the ceramide increase in GMC, which is significant to effect on the kidney injury of the rats with obstructive jaundice, by the means of inducing GMC apoptosis and decreasing the activity of PC-PLD.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Icterícia Obstrutiva
/
Células Mesangiais
/
Glomérulos Renais
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China