A novel method for efficient generation of transfected human osteoclasts.
Calcif Tissue Int
; 80(2): 132-6, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17308995
ABSTRACT
Mature osteoclasts and their precursors are notoriously difficult to transfect using nonviral approaches, a limitation that represents a major technical obstacle in the study of osteoclast biology. Here, we describe a simple electroporation method using Amaxa Nucleofector technology that results in efficient transfection of human blood-derived osteoclast precursors, which can be differentiated in subsequent culture to generate mature osteoclasts that retain expression of the transgene. Moreover, since these osteoclasts maintain the ability to resorb dentine, this technique could prove useful for assessing the role of specific genes/proteins in osteoclast function.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoclastos
/
Transfecção
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Calcif Tissue Int
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Reino Unido