Sox15 and Fhl3 transcriptionally coactivate Foxk1 and regulate myogenic progenitor cells.
EMBO J
; 26(7): 1902-12, 2007 Apr 04.
Article
em En
| MEDLINE
| ID: mdl-17363903
ABSTRACT
The regulation of myogenic progenitor cells during muscle regeneration is not clearly understood. We have previously shown that the Foxk1 gene, a member of the forkhead/winged helix family of transcription factors, is expressed in myogenic progenitor cells in adult skeletal muscle. In the present study, we utilize transgenic technology and demonstrate that the 4.6 kb upstream fragment of the Foxk1 gene directs beta-galactosidase expression to the myogenic progenitor cell population. We further establish that Sox15 directs Foxk1 expression to the myogenic progenitor cell population, as it binds to an evolutionarily conserved site and recruits Fhl3 to transcriptionally coactivate Foxk1 gene expression. Knockdown of endogenous Sox15 results in perturbed cell cycle kinetics and decreased Foxk1 expression. Furthermore, Sox15 mutant mice display perturbed skeletal muscle regeneration, due in part to decreased numbers of satellite cells and decreased Foxk1 expression. These studies demonstrate that Sox15, Fhl3 and Foxk1 function to coordinately regulate the myogenic progenitor cell population and skeletal muscle regeneration.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Fatores de Transcrição
/
Transcrição Gênica
/
Proteínas de Grupo de Alta Mobilidade
/
Músculo Esquelético
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Peptídeos e Proteínas de Sinalização Intracelular
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Fatores de Transcrição Forkhead
Limite:
Animals
Idioma:
En
Revista:
EMBO J
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos