Electrochemical properties of cytochroms P450 using nanostructured electrodes: direct electron transfer and electro catalysis.
J Inorg Biochem
; 101(5): 859-65, 2007 May.
Article
em En
| MEDLINE
| ID: mdl-17376532
ABSTRACT
The present study demonstrates direct electron transfer between cytochromes P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14alpha-demethylase (CYP51b1) on the one hand and screen-printed graphite electrodes, modified with gold nanoparticles and didodecyldimethylammonium bromide (DDAB) on the other. Electro detection of heme proteins was possible when 2-200 pmol P450/electrode were adsorbed on the surface of nanostructured electrochemical interfaces. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, and lanosterol) and with P450 inhibitor (ketoconazole) were analyzed using cyclic voltammetry (CV), square wave voltammetry (SWV) differential pulse voltammetry (DPV), and amperometry.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Sistema Enzimático do Citocromo P-450
/
Nanopartículas Metálicas
Limite:
Animals
Idioma:
En
Revista:
J Inorg Biochem
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Federação Russa