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Manganese speciation in human cerebrospinal fluid using CZE coupled to inductively coupled plasma MS.
Michalke, Bernhard; Berthele, Achim; Mistriotis, Panos; Ochsenkühn-Petropoulou, Maria; Halbach, Stefan.
Afiliação
  • Michalke B; GSF-National Research Center for Environment and Health, Institute of Ecological Chemistry, Neuherberg, Germany. bernhard.michalke@gsf.de
Electrophoresis ; 28(9): 1380-6, 2007 May.
Article em En | MEDLINE | ID: mdl-17377947
The neurotoxic effects of manganese (Mn) at elevated concentrations are well known. This raises the question, which of the Mn species can cross neural barriers and appear in cerebrospinal fluid (CSF). CSF is the last matrix in a living human organism available for analysis before a compound reaches the brain cells and therefore it is assumed to reflect best the internal exposure of brain tissue to Mn species. A previously developed CE method was modified for separation of albumin, histidine, tyrosine, cystine, fumarate, malate, inorganic Mn, oxalacetate, alpha-keto-glutarate, nicotinamide-dinucleotide (NAD), citrate, adenosine, glutathione, and glutamine. These compounds are supposed in the literature to act as potential Mn carriers. In a first attempt, these compounds were analyzed by CZE-UV to check whether they are present in CSF. The CZE-UV method was simpler than the coupled CZE-inductively coupled plasma (ICP)-dynamic reaction cell (DRC)-MS method and it was therefore chosen to obtain a first overview information. In a second step, the coupled method (CZE-ICP-DRC-MS) was used to analyze, in detail, which of the compounds found in CSF by CZE-UV were actually bound to Mn. Finally, 13 Mn species were monitored in CSF samples, most of them being identified: Mn-histidine, Mn-fumarate, Mn-malate, inorganic Mn, Mn-oxalacetate, Mn-alpha-keto glutarate, Mn-carrying NAD, Mn-citrate and Mn-adenosine. By far the most abundant Mn species was Mn-citrate showing a concentration of 0.7 +/- 0.13 microg Mn/L. Interestingly, several other Mn species can be related to the citric acid cycle.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Compostos de Manganês / Eletroforese Capilar / Manganês Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Electrophoresis Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Alemanha
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Compostos de Manganês / Eletroforese Capilar / Manganês Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Electrophoresis Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Alemanha