Processing of chimeric introns in dicot plants: evidence for a close cooperation between 5' and 3' splice sites.
Nucleic Acids Res
; 20(1): 75-81, 1992 Jan 11.
Article
em En
| MEDLINE
| ID: mdl-1738607
ABSTRACT
Splice sites of vertebrate introns are generally not recognized in plant cells. Several lines of evidences have led to the proposal that the mechanism of 3' splice site selection differs in plants and animals (K. Wiebauer, J.J. Herrero, and W. Filipowicz, Mol. Cell. Biol. 82042-2051, 1988). To gain a better insight into the mechanistic differences between plant and animal splicing, we constructed chimeric introns consisting partly of dicotyledonous plant and partly of animal intron sequences. Splicing of these chimeric introns was analyzed in transiently transfected tobacco protoplasts. The results show that there are no principal sequence or structural differences between the 3' splice regions of plants and animals. Furthermore, evidence is provided that cooperation between 5' and 3' splice sites takes place and influences their mutual selection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas
/
RNA Mensageiro
/
Íntrons
/
Splicing de RNA
/
Plantas Geneticamente Modificadas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Áustria