The interaction of N-acetylhexosaminidase with insolubilized concanavalin A.
Biochem J
; 163(2): 247-51, 1977 May 01.
Article
em En
| MEDLINE
| ID: mdl-17397
ABSTRACT
The specific interaction between human N-acetylhexosaminidase and concanavalin A was evaluated with respect to temperature, time, pH and concentration of specific ligand in incubation mixtures containing the enzyme and insolubilized lectin. Elution of the enzyme from insolubilized concanavalin A is dependent on both temperature and concentration of alpha-methyl mannoside. Conditions for a high yield of the enzyme from chromatography on insolubilized concanavalin A are described.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Concanavalina A
/
Hexosaminidases
Limite:
Humans
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1977
Tipo de documento:
Article