Specialized techniques for site-directed mutagenesis in cyanobacteria.
Methods Mol Biol
; 362: 155-71, 2007.
Article
em En
| MEDLINE
| ID: mdl-17417008
ABSTRACT
Synechococcus elongatus PCC 7942 is an excellent model system for studying the molecular mechanism of the circadian clock in cyanobacteria. The "plastic" genetic characteristics of this organism have facilitated the development of various methods for mutagenesis of its chromosome. These methods are based on homologous recombination between the chromosome and foreign DNA, introduced to the cyanobacteria by either transformation or conjugation. Here we describe different approaches to mutagenize the chromosome of S. elongatus, including insertional mutagenesis, hit-and-run allele replacement, rps12-mediated gene replacement, and regulated expression of genes from ectopic sites, the neutral sites of the S. elongatus genome.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mutagênese Sítio-Dirigida
/
Synechococcus
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos