Synthesis of alpha-chymotrypsin/polymer composites by a reverse micelle/gas antisolvent method.
Colloids Surf B Biointerfaces
; 59(1): 11-5, 2007 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-17532613
ABSTRACT
Alpha-chymotrypsin (CT)/polyvinylpyrrolidone (PVP) composites was synthesized by combination of reverse micelles and CO(2). In this method, the two reverse micellar solutions containing CT and PVP, respectively, were first mixed, then compressed CO(2) was used as an antisolvent to precipitate the CT and PVP simultaneously and CT/PVP composites were successfully prepared. The morphology of the obtained CT/PVP composites was characterized by transmission electron microscopy (TEM). The FTIR spectra of the composites showed that there was interaction between CT and PVP. The storage activity of the enzyme immobilized on the polymer by this method was higher than that of the pure enzyme. This method has some advantages and can be easily applied to the synthesis of some other enzyme/polymer composites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quimotripsina
/
Povidona
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Assunto da revista:
QUIMICA
Ano de publicação:
2007
Tipo de documento:
Article