Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS).
J Zhejiang Univ Sci B
; 8(5): 331-7, 2007 May.
Article
em En
| MEDLINE
| ID: mdl-17542061
ABSTRACT
Seeds of a Chinese traditional medicine plant, Cochinchina momordica were used in the present study for the improvement of influenza vaccine (H5N1) in chicken. Crude extraction from Cochinchina momordica seed (ECMS) was obtained by ethanol extraction method. In experiment No. 1, two weeks old chickens were immunized with influenza vaccine (H5N1) alone or combined with ECMS (5, 10, 20, 40 and 80 microg/dose). Serum IgG antibody levels (by ELISA) as well as effects on daily weight gain were measured on 0, 7, 14 and 28th day after immunization. Results revealed that all ECMS groups numerically increased the antibody levels while 10 and 20 microg/dose groups significantly (P<0.05) enhanced total IgG antibody on day 28, when compared with control. Average daily weight gain was also significantly higher in 20 microg/dose ECMS group. Adjuvant effect was also confirmed in experiment No. 2 when chickens were immunized with 20 microg/dose ECMS and antibody titer was measured through hemagglutination inhibition (HI). It is concluded that ECMS has potential to improve the immune responses and deserve further study as an adjuvant.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Medicamentos de Ervas Chinesas
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Vacinas contra Influenza
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Magnoliopsida
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Virus da Influenza A Subtipo H5N1
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Influenza Aviária
Limite:
Animals
Idioma:
En
Revista:
J Zhejiang Univ Sci B
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
CHINA
/
CN
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REPUBLIC OF CHINA